In this study, we used multilocus allozyme markers and maternity exclusion analysis to estimate dispersal distances in an old-growth population of a wind-dispersed conifer, Pinus palustris. Of 2,618 seeds analyzed, 11.3% did not have maternal parents within 75 m, 7.8% had a single possible maternal parent, and 80.9% had more than one possible maternal parent within 75 m. Using a variety of methods, a consensus dispersal curve was obtained and can be described as a broad mono-modal curve with peak dispersal at a distance of 35–60 m from the parent. Total gene flow beyond 75 m was calculated to be 54%, suggesting that a substantial percentage of gametes dispersed beyond 75 m. Results indicate that seeds of longleaf pine have the potential to disperse greater distances than previously reported, which may explain, in part, the low levels of genetic structure in this old-growth population.